Oocyte/ Embryo Cryopreservation

 

Documenting cryopreservation is a two-step process. The process enables all of the information from Lab Notes to be seamlessly imported to the cryopreservation log for the practice. The first step is selecting the oocytes/embryos from Lab Notes for cryopreservation. The second is creating locations (straw or vial numbers) in the system of the cryopreservation record for the samples.

Selecting an Oocyte/Embryo for Cryopreservation from Lab Notes

 

 

Selecting embryos/oocytes for cryopreservation may be accomplished through either bulk or individual grading.  To cryo individually click on the cell that contains the oocyte that you wish to freeze to make your disposition. 

 

In this example, we are freezing an oocyte prior to insemination, so we select the Cryo button under both Dispositions. Cryo and oocyte cryo. Click the Commit button to save.

The selected cell now displays the blue cryo disposition. 

 

Use the Cryopreservation tab to Record Cryo Straw/Vial Number & Tank Location

Once you have selected oocytes/embryos for cryopreservation, go to the Cryopreservation tab.

 

 

First, from the Cryopreservation tab use the pulldown to select Technician and enter the Cryo date. Oocytes/embryos designated for cryo are displayed in the listed on the left side under Embryos selected for cryo. 

 

Click inside the cell # of devices and enter how many straws are needed. Select the purple  'Go' play button. Then complete the information by filling in Device, label, Tank #, Cane label,  Vane color, and Device Color. 

 

You are able to enter the number of straws/vials needed and then enter in the device, label, tank #, cane label, cane color, and device color. Next, select the purple play button. Or you can create all straws then after the straws are created edit each cell with the correct information. 

 

 

Use the pull-down menu for Vial # in the Embryos Selected for Cryo portal to assign a Straw/Vial number to each sample. If freezing individually, assign one device label to each. If freezing together, assign the device label to the appropriate multiple samples. 

 

 

 

To customize the vial #, click inside the cell and free text the new information. 

All of this information now resides in the Cryologs/Specimen Log.  You are also able to recryo embryos and oocytes from this page by selecting the recryo date and technician.

 

**All manuals are compliant with HIPPA regulations.  No patient information has been released.  All screenshots above were taken from a test clinic with fake patient information.